温和冷应激及复温对牦牛卵巢颗粒细胞的影响

doi:10.11843/j.issn.0366-6964.2015.05.008

Abstract

Abstract:

The cellular effects of hypothermia has been recognized a complex process including a stress response to cold and subsequent rewarming.In this study，real-time fluorescence quantitative PCR and Western blot techniques were used to observe the dynamic expression of CIRBP，HSP70 and P53 in the yak ovarian granulosa cells.Cells were preincubated at 25 ℃ for 1 and 5 d,and rewarmed at 37 ℃ for 1,2,4,8 and 24 h.The results showed that hypothermia and subsequent rewarming affected cell biology and induced a cellular stress response.The expression of CIRBP was increased at 25 ℃ already at day 1 and returned to basal level upon rewarming after 24 h.While the expression of HSP70 was gradually increased upon rewarming at 37 ℃，maximal expression was occurred at 8 h after rewarming and followed by decline.Moreover the maximal expression of P53 was appeared at 4 h after rewarming and followed by a decline.Additionally，the observation that P53 mRNA was significantly decreased，a process that accompanied a maximal expression of CIRBP and HSP70 mRNA，suggested that the expression of CIRBP mRNA and protein was increased upon exposure to hypothermia，and HSP70 was induced upon rewarming.Overall，the CIRBP and HSP70 were shown to play a role in protecting cells from the deleterious effects of stress and apoptosis.These findings would bring new insights into the potential beneficial effects of mild hypothermia and rewarming used in various research and therapeutical fields.

CLC Number:

S823.8⁺5.2

YANG Kun，ZHANG Qian，LI Gu-yue，PAN Yang-yang，YU Si-jiu，HE Jun-feng，CUI Yan. Effects of Mild Cold Shock Followed by Warming Up at 37 ℃ on the Ovarian Granulosa Cellular Stress Response of the Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 738-745.

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Effects of Mild Cold Shock Followed by Warming Up at 37 ℃ on the Ovarian Granulosa Cellular Stress Response of the Yak

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